Phospho-FADD (Ser194) Recombinant Rabbit Monoclonal Antibody

SKU: orb1499265

Description

Research Area

Cancer Research, Fas-associated protein with death domain, Necroptosis Pathway

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	R3D7
Immunogen	KLH conjugated synthetic peptide derived from human phospho-FADD (Ser194)
Target	FADD
Molecular Weight	23 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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FADD_HUMAN; FAS-associated death domain protein; FAS-associating death domain-containing protein; MORT1; GIG3; GIG 3; IMD90; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity;

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Phospho-FADD (Ser194) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): HeLa, (+): HeLa + nocodazole, (500 ng/ml, 16 h), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 23 kDa, Observed MW: 23 kDa.

Quick Database Links

Gene Symbol

FADD

UniProt

Q13158

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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