Phospho-Histone H2A.X (Ser139) (1B3) rabbit mAb APC conjugate Antibody

SKU: orb1946204

Description

Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as gH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in response to double-strand breaks formed during apoptosis. However, physiological phosphorylation of Histone H2AX occurs when double-strand DNA breaks are formed during meiosis and V(D)J recombination. A549 and DU145 cell lines have been found to have higher expression levels of phosphorylated Histone H2AX compared to Jurkat, MCF-7, or HL-60 cell lines.

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Tested Applications	FC
Dilution Range	For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	HisH2AXS139-1B3
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho-histone H2A.X
Purification	Protein A+G
Conjugation	APC

Storage & Handling

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Storage	2-8°C
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.09% NaN3, 0.2% rAlbumin
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H2AFX, H2AX, γH2AX, gamma-H2AX

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Phospho-Histone H2A.X (Ser139) (1B3) rabbit mAb APC conjugate Antibody

Flow cytometric analysis of 293T cells, untreated and unstained as negative control (blue) or untreated and stained (red) or treated with UV and PMA and stained (green) using Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1B3 APC conjugate, Cat. #orb1946204.

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UniProt

P16104

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No UniProt data available

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Protocol Information

Flow Cytometry

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