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Phospho-IKB Alpha (Ser32 / Ser36) Recombinant Rabbit Monoclonal Antibody
SKU: orb1499271
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Description
Research Area
NF-kB Signalling Pathway, TLR Signaling Pathway
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | R8D3 |
| Immunogen | KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36) |
| Target | NFKBIA |
| Molecular Weight | 35 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN; IκB-α; IκBα.
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): C6, (+): C6 + Calyculin A (100nM, 30 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 36 kDa, Observed MW: 36 kDa.
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): HeLa, (+): HeLa + Human α-TNF, (20 ng/ml, 30 min), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 36 kDa, Observed MW: 36 kDa.
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline, phosphatase (50 U/ml) at 37°C for 1 hour, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499271.
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50, U/ml) at 37°C for 1 hour, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499271.
Quick Database Links
Gene Symbol
NFKBIA
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Phospho-IKB Alpha (Ser32 / Ser36) Recombinant Rabbit Monoclonal Antibody (orb1499271)
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