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Phospho-IKK beta (Ser476) Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ICC |
|---|---|
| Dilution range | ICC/IF=1:50-200 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Porcine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human IKK beta around the phosphorylation site of Ser476 SM(p-S)QQ |
| Target | IKBKB |
| Molecular Weight | 87 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IKK beta (Ser476)) polyclonal Antibody, Unconjugated (orb5526) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
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Phospho-IKK beta (Ser476) Rabbit Polyclonal Antibody (orb5526)
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