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Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody
SKU: orb559184
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:500-1000, ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Sheep |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 28C3 |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human IRF3 around the phosphorylation site of Ser386 S(p-S)LE |
| Target | IRF3 |
| Molecular Weight | 47 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−IRF3(S386); IRF3(phospho S386); IRF3(phospho Ser386); p-IRF3(Ser386); p-IRF3(S386); Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729.
Quality Guarantee
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MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-IRF3 (S386)) monoclonal Antibody, Unconjugated (orb559184) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
IRF3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (orb559184)
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