Phospho-MARCKS (Ser167/170) (C9) rabbit mAb Antibody

SKU: orb1946011

Description

MARCKS (myristoylated alanine-rich C kinase substrate) is a major PKC substrate expressed in all eukaryotic cells(1,2). It binds to and cross-links actin filaments to serve as a bridge between Ca2+/calmodulin and PKC signaling and attenuates phosphatidylinositol 4,5-bisphosphate plasma membrane signaling (3). MARCKS is involved with cell mobility, phagocytosis, membrane traffic, cell adhesion, and mitogenesis. Ser159, 163, 167 and 170 of MARCKS are phosphorylated by PKC in response to cell groeth and cellular stress (4). MARCKs phosphorylation is believe to induce its tranlocation from plasma membrane to cytoplasm.

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	MARCKSS167170-C9
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, PKCSL, 80 kDa protein light chain, MACS, PRKCSL

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Phospho-MARCKS (Ser167/170) (C9) rabbit mAb Antibody

Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV + TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 μg/mL. Cat. #orb1946011.

Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.1 ug/mL. Cat. #orb1946011.

Flow cytometric analysis of 293T cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 μg/mL. Cat. #orb1946011.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using 0.1 ug/mL of Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 μg/mL (Cat. #orb1946011) or Company C antibody at 0.5 ug/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P29966

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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