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Phospho-MeCP2(S423) Antibody
SKU: orb1931066
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P-Leica - 1:500, WB - 1:500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Monkey |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 52441 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
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Immunohistochemical analysis of paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Western blot analysis of lysates from C6 cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
Western blot analysis of lysates from Jurkat cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
Western blot analysis of lysates from Neuro-2a cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Phospho-MeCP2(S423) Antibody (orb1931066)
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