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Phospho-MEK1 (Thr292) Recombinant Rabbit Monoclonal Antibody

SKU: orb559178

Description

Phospho-MEK1 (Thr292) Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology, MAPK/ERK Signalling Pathway, Tyrosine Kinases

Images & Validation

Tested ApplicationsICC, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.3F10
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr292 PR(p-T)PG
TargetMAP2K2
Molecular Weight43 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MEK1(T292); MEK1(phospho T292); p-MEK1(phospho T292); MEK1(phospho Thr292); MEK1(phospho T292); p-MEK1(Thr292); p-MEK1(T292); Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1; Protein kinase mitogen activated kinase 1(MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN.
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Phospho-MEK1 (Thr292) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: U87MG (Human) Cell Lysate at 30 ug, Lane 2: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-MEK1 (Thr292) (orb559178) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 43 kD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

Phospho-MEK1 (Thr292) Recombinant Rabbit Monoclonal Antibody (orb559178)

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50 μl
$ 330.00
100 μl
$ 490.00
DispatchUsually dispatched within 5-10 working days
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