Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb Antibody

SKU: orb1946168

Description

MKK3 and MKK6 are closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). Phospho MKK3 and phospho MKK6 both phosphorylate and activate p38. p38 phosphorylation dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Phospho MKK3 and phospho MKK6 activation occurs through phosphorylation at S189 and T222 on MKK3 (2) and S207 and T211 on MKK6 (4,5).

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	MKK3S189MKK6S207-D3
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK2, MAP2K3, MEK3, PRKMK3, SKK2

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Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb Antibody

Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 μg/mL Phospho-MKK3 (Ser189) MKK6 (S207) antibody MKK3S189MKK6S207-D3. Cat. #orb1946168.

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 0.1μg/mL. Cat. # orb1946168.

MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #orb1946168.

Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV + TPA (green) using 0.5 μg/mL of Phospho-MKK3 (Ser189) /MKK6 (Ser207) antibody MKK3S189MKK6S207-D3 Cat. #orb1946168.

Quick Database Links

UniProt

P46734

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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