Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody

SKU: orb6993

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1μg /Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr696 RS(p-T)QG
Target	PPP1R12A
Molecular Weight	113 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MYPT1(phospho Thr696); MYPT1(phospho T696); Myosin Phosphatase(phospho T696); Myosin Phosphatase(phospho Thr696); p-MYPT1(Thr696); Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; MYPT 1; MYPT1; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase myosin binding subunit; MYPT1_HUMAN; Myosin phosphatase-targeting subunit 1; Protein phosphatase myosin-binding subunit.

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Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody

Blank control (blue): Hela cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-MYPT1 (Thr696) antibody (orb6993), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: 293T (human) cell Lysate at 40 ug, Primary: Anti-p-MYPT1 (Thr696) (orb6993) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113kD, Observed band size: 120 kD.

Sample: BRL-3A (Rat) cell Lysate at 40 ug, Primary: Anti-p-MYPT1 (Thr696) (orb6993) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113kD, Observed band size: 120 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-Phospho-MYPT1 (Thr696) (orb6993) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kDa, Observed band size: 125 kDa.

Quick Database Links

Gene Symbol

PPP1R12A

UniProt

O14974

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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