Phospho-NDRG1 (Thr346) (F5) rabbit mAb Antibody

SKU: orb1946264

Description

N-Myc down-regulated gene 1 (NDRG1) has been reported to be a direct transcriptional target of p53. NDRG1 appears to play a necessary, but not sufficient, role in apoptosis, though its exact mechanism of action remains unknown. NDRG1 expression is elevated in non-small cell lung cancer cells, promoting cancer growth and reducing cytotoxicity to certain anti-cancer drugs. NDRG1 is also elevated in solid tumors and is recognized as a negative prognostic indicator in breast cancer. Elevated NDRG1 expression is correlated with disease recurrence and metastasis in breast cancer. NDRG1 is phosphorylated by Sgk1, which itself is activated by mTORC2. Phosphorylation of NDRG1 at Thr346 promotes cellular differentiation in adipocytes.

Images & Validation

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Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	NDRG1T346-F5
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr346 of human phospho NDRG1
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, RTP, Rit42

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Phospho-NDRG1 (Thr346) (F5) rabbit mAb Antibody

Peptide blocking flow cytometric analysis of THP1 cells secondary antibody only negative control (light blue) or untreated (orange) using isotype control antibody #orb1946241 or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 at 0.05 μg/mL. Cat. #orb1946264.

Flow cytometric analysis of THP1 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 at 0.05 μg/mL. Cat. #orb1946264.

Western blot analysis of C2C12 cell extract untreated or treated with LY294002 using 0.05 ug/mL Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 Cat. #orb1946264.

Western blot analysis of C2C12 cell extract untreated or treated with LY294002 using 0.05 μg/mL Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 Cat. #orb1946264 or Company C antibody at 0.07 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

Q92597

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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