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Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

SKU: orb6607
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Description

Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human p63 around the phosphorylation site of Ser455 IQ(p-S)PS
TargetTP63
Molecular Weight77 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

P63_HUMAN; Tumor protein 63; TP63; Chronic ulcerative stomatitis protein(CUSP); Keratinocyte transcription factor KET; Transformation-related protein 63(TP63); Tumor protein p73-like(p73L); p40; p51; KET; P63; P73H; P73L; TP73L; AIS; LMS; NBP; RHS; p40; p51; EEC3; OFC8; SHFM4; TP53L; p53CP; TP53CP; B(p51A); B(p51B); P51A; TAp63gamma; P51B; TAp63alpha; P51delta; TAp63delta; P51delNalpha; deltaNp63 alpha; P51delNbeta; deltaNp63 beta; P51delNgamma; deltaNp63gamma; p40-deltaNp63; p40-deltaNp63;

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Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-p63 (Ser455)) Polyclonal Antibody, Unconjugated (orb6607) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-p63 (Ser455)) Polyclonal Antibody, Unconjugated (orb6607) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human mammary gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-p63 (Ser455)) Polyclonal Antibody, Unconjugated (orb6607) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p63 (Ser455) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-p63 (Ser455)) Polyclonal Antibody, Unconjugated (orb6607) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-p63 (Ser455) Rabbit Polyclonal Antibody (orb6607)

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50 μl
$ 320.00
100 μl
$ 490.00
200 μl
$ 580.00
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