Phospho-PLCg1 (Tyr783) (C4) rabbit mAb Antibody

SKU: orb1946203

Description

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositolphosphate to inositol triphosphate and diacylglycerol. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate diacylglycerols (DAGs) and water-soluble phosphorylated derivatives, such as inositol 1,4,5-triphosphate (IP3).„ÄÄWithin the PLC family, PLCg is the only member that contains SH2 and SH3 domains, necessary for phospho PLCg activation. Phospho PLCg, upon activation, can interact with receptor tyrosine kinases.

Images & Validation

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Item 1 of 4

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	PLCG1Y783-C4
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr783 of human phospho PLCg1.
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC1

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Phospho-PLCg1 (Tyr783) (C4) rabbit mAb Antibody

Peptide blockage flow cytometric analysis of Hela cells secondary antibody only negative control (blue) treated with imatinib (red) treated with pervanadate (green) treated with PV + blocked with non-phospho- peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using Phospho-PLCγ1 (Tyr783) antibody at 0.05 μg/mL PLCg1Y783-C4. Cat. #orb1946203.

PLCg1Y783-C4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4 (green) Cat. #orb1946203.

Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 μg/mL Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4. Cat. #orb1946203.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4 (Cat. #orb1946203) or Company C antibody at 0.1 ug/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P19174

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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