Phospho-PLK1 (Thr210) Recombinant Rabbit Monoclonal Antibody

SKU: orb559133

Description

Research Area

Cancer Research, Cell Biology, Cell Cycle, Phosphatases

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	2A10
Immunogen	KLH conjugated synthesised phosphopeptide derived from human PLK1 around the phosphorylation site of Thr210 KK(p-T)L
Target	plk1
Molecular Weight	68 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PLK1(T210); PLK1(phospho T210); p-PLK1(phospho T210); PLK1(phospho-Thr210); PLK1(phospho Thr210); p-PLK1(Thr210); p-PLK1(T210); Polo-Like Kinase(phospho T210); PLK 1; PLK; polio like kinase; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase; STPK 13; STPK13; Polo like kinase kinase; Cell cycle regulated protein kinase; PLK-1; PLK1_HUMAN.

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Phospho-PLK1 (Thr210) Recombinant Rabbit Monoclonal Antibody

Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-Phospho-PLK1 (T210) antibody (orb559133), dilution: 1:50, Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.