Phospho-RAF1 (Ser43) Recombinant Rabbit Monoclonal Antibody

SKU: orb526683

Description

Research Area

Apoptosis, Cancer Research, Cell Biology, Cell Cycle, Kinases, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	4G2
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human RAF1 around the phosphorylation site of Ser43 RA(p-S)DD
Target	RAF1
Molecular Weight	71 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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RAF1(phospho S43); phospho-Raf1(S43); p-Raf1(S43); phospho-Raf1(S43); murine leukemia viral(v-raf-1) oncogene homolog 1(3611-MSV); v-raf-1 murine leukemia viral oncogene homolog 1; cRaf; C-RAF; proto-oncogene c-RAF; 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; MGC102375; Raf-1; Raf 1; v-Raf; c Raf; Craf 1 transforming gene; Craf1 transforming gene; EC 2.7.11.1; Murine sarcoma 3611 oncogene 1; RAF; Raf proto oncogene serine/threonine protein kinase; vraf1 murine leukemia viral oncogene homolog 1; c-Raf.

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Phospho-RAF1 (Ser43) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: NIH/3T3 cell lysate, Lane 2: 293T cell lysate, Primary: Anti-Phospho-Raf1 (Ser43) (orb526683) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 71 kD, Observed band size: 71 kD.