Phospho-SIRT1 (Ser27) Recombinant Rabbit Monoclonal Antibody

SKU: orb1499327

Description

Research Area

Host-Virus Interaction, Immunology, Obesity, Virology, p53 Signaling Pathway

Images & Validation

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Item 1 of 1

Tested Applications	ICC, WB
Dilution Range	WB=1:500-2000, ICC/IF=1:50-100
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	R4C3
Immunogen	KLH conjugated synthetic peptide derived from human phospho-SIRT1 (Ser27)
Target	SIRT1
Molecular Weight	81 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SIR2L1; NAD-dependent protein deacetylase sirtuin-1; EC:2.3.1.286; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1(hSIR2); SirtT1 75 kDa fragment; SIR1_HUMAN;

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Phospho-SIRT1 (Ser27) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1-2: HEK-293+CA (-) (+), 3-4: Jurkat+CA (-) (+), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 82 kDa, Observed MW: 120 kDa.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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