Phospho-SP1 (Thr739) Rabbit Polyclonal Antibody

SKU: orb186031

Description

Research Area

Cell Biology, DNA and RNA, DNA and RNA Research, Mitochondrial Biogenesis, Nuclear Signaling Pathway, Zinc Finger Protein, mitochondrial transcription

Images & Validation

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Item 1 of 1

Tested Applications	ICC
Dilution Range	ICC/IF=1:50-200
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Monkey

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human SP1 around the phosphorylation site of Thr739 TA(p-T)PS
Target	SP1
Molecular Weight	81 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SP1(phospho T739); SP1(phospho-Thr739); SP1(phospho-T739); p-SP1(phospho T453); p-TSFP1(phospho T453); Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP1_HUMAN.

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Phospho-SP1 (Thr739) Rabbit Polyclonal Antibody

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-SP1 (Thr739)) polyclonal Antibody, Unconjugated (orb186031) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

SP1

UniProt

P08047

UniProt Details

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No UniProt data available

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Protocol Information

ICC

Immunocytochemistry

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