Physalin B

SKU: orb1684320

Description

Physalin B, a bioactive compound from Solanaceae plants, exhibits broad anti-inflammatory and antitumor activities. It attenuates acute lung injury by suppressing NF-κB/NLRP3 via PI3K/Akt and inhibits VSMC proliferation via the Nrf2 pathway. Research applications include studies in inflammation, vascular remodeling, and oncology using in vitro and in vivo models.

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	23133-56-4
MW	510.53
Purity	98.40%
Formula	C₂₈H₃₀O₉
SMILES	[H][C@@]12C(=O)[C@]34O[C@]11[C@@](C)(OC(=O)[C@@]1(O)CC[C@@]1([H])[C@@]3([H])CC=C3CC=CC(=O)[C@]13C)[C@H]1C[C@]2(C)[C@@]([H])(CO4)C(=O)O1
Target	Autophagy,Nrf2,NOD,Akt,PI3K,NF-κB,Apoptosis

Bioactivity

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In Vitro

Physalin B inhibited the viability of HCT116 cells with an IC50 value of 1.35 μmol/L. Treatment of the cells with physalin B (2.5-10 μmol/L) induced apoptosis and the cleavage of PARP and caspase-3. Meanwhile, physalin B treatment induced autophagosome formation, and accumulation of LC3-II and p62, but decreased Beclin 1 protein level. Marked changes of microtubules and F-actin microfilaments were observed in physalin B-treated cells, which led to the blockage of co-localization of autophagosomes and lysosomes. Physalin B treatment dose-dependently increased the phosphorylation of p38, ERK, and JNK in the cells, whereas the p38 inhibitor SB202190, ERK inhibitor U0126, or JNK inhibitor SP600125 could partially reduce physalin B-induced PARP cleavage and p62 accumulation. Moreover, physalin B treatment dose-dependently increased mito-ROS production in the cells, whereas the ROS scavenger NAC could reverse physalin B-induced effects, including incomplete autophagic response, accumulation of ubiquitinated proteins, changes of microtubules and F-actin, activation of p38, ERK and JNK, as well as cell death and apoptosis. Physalin B induces mito-ROS, which not only inhibits the ubiquitin-proteasome pathway but also induces an incomplete autophagic response in HCT116 cells in vitro.

Storage & Handling

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Storage	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Akt, Antifection, Apoptosis, Bcl-2, Caspase, NSC287088, NSC-287088, NOD, NFkB, NF-kB, NFκB, NF-κB, NSC 287088, Nrf2, PI3K, Physalin B

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Compound Identifiers

PubChem CID

11613161 ↗

Key Properties

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No computed properties available.

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Physalin B

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

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Protocol Information

Physalin B (orb1684320)

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