Piccolo Antibody (PerCP)

SKU: orb147929

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Description

Mouse monoclonal to Piccolo (PerCP). Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1..

Images & Validation

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Tested Applications	ELISA, ICC, IF, WB
Dilution range	WB (1:1000), ICC/IF (1:100)
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b Kappa
Clone No.	6H9-B6
Immunogen	Full length recombinant protein (GenBank ID: BC001304.1)
Target	Piccolo
Molecular Weight	550kDa
Purification	Protein G Purified
Conjugation	PerCP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Protein piccolo, Aczonin, PCLO, Presynaptic cytomatrix protein, Multidomain presynaptic cytomatrix protein, Piccolo (presynaptic cytomatrix protein), PCLO_HUMAN, ACZ, Pico, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Piccolo mouse homolog of Pico

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.

Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.

Quick Database Links

Gene Symbol

Piccolo

UniProt

Q9QYX7

NCBI Gene ID

26875

RefSeq (Protein):NP_001104266.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001104266.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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