Pig Epidermal Growth Factor Receptor (EGFR) ELISA Kit

SKU: orb782262

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor Receptor(EGFR).

Research Area

Neuroscience; Stem Cell & Developmental Biology; Cell Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig EGFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Porcine
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.25 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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ErbB-1 ELISA Kit, ErbB1 ELISA Kit, ER1 ELISA Kit, ERB-B1 ELISA Kit, MENA ELISA Kit, HER1 ELISA Kit, Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian ELISA Kit, Proto-oncogene c-ErbB-1 ELISA Kit, Receptor tyrosine-protein kinase erbB-1 ELISA Kit

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Pig Epidermal Growth Factor Receptor (EGFR) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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