Pig Selectin, Endothelium (SELE) ELISA Kit

SKU: orb779530

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Endothelium(SELE).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig SELE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SELE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig SELE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig SELE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Porcine
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.65 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CD62E ELISA Kit, CD62-E ELISA Kit, E-Selectin ELISA Kit, ELAM ELISA Kit, ELAM1 ELISA Kit, ESEL ELISA Kit, SEL-E ELISA Kit, E-LAM ELISA Kit, E-LAM1 ELISA Kit, E-SEL ELISA Kit, LECAM2 ELISA Kit, Endothelial Leukocyte Adhesion Molecule 1 ELISA Kit, CD62 Antigen-Like Family Member E ELISA Kit

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Pig Selectin, Endothelium (SELE) ELISA Kit

Quick Database Links

UniProt

P98110

UniProt Details

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No UniProt data available

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Datasheet

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Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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