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PKC ε rabbit pAb Antibody
SKU: orb769656
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Description
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−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC epsilon. AA range:688-737 |
| Molecular Weight | 83 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-PRKCE antibody, anti-PKCE antibody, anti-Protein kinase C epsilon type antibody, anti-nPKC-epsilon antibody
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Western Blot analysis of 453 cells using PKC ε Polyclonal Antibody diluted at 1:500.
Western blot analysis of lysates from HeLa, Jurkat, and 3T3 cells, treated with PMA 125ng/ml 30', using PKC epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PKC ε rabbit pAb Antibody (orb769656)
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