Plant Indole 3 Acetic Acid (IAA) ELISA Kit

SKU: orb782547

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Indole 3 Acetic Acid(IAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indole 3 Acetic Acid(IAA).

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant IAA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Plant
Sample Types	plant tissues
Assay Type	Competitive
Assay Time	2.5h
Range	3.13-200 ng/mL
Sensitivity	0.88 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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IA-A ELISA Kit, Indolylacetic Acid ELISA Kit, Indoleacetic Acid ELISA Kit, Heteroauxin ELISA Kit

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Plant Indole 3 Acetic Acid (IAA) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Plant (1)

Sohail Mumtaz 1, Rida Javed 2, Juie Nahushkumar Rana 2, Madeeha Iqbal 2, Eun Ha Choi Pulsed high power microwave seeds priming modulates germination, growth, redox homeostasis, and hormonal shifts in barley for improved seedling growth: Unleashing the molecular dynamics Free Radic Biol Med, (2024)

PubMed: 38901500

Applications

ELISA

Reactivity

Plant