Melanoma gp100 Antibody / PMEL17

SKU: orb534214

Description

PMEL17/Melanoma gp100/SILV plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. [UniProt]

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the Melanoma gp100 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	MSSG95-2
Immunogen	A portion of amino acids 376-502 from the human protein was used as the immunogen for the Melanoma gp100 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of human melanoma with Melanoma gp100 antibody (clone MSSG95-2). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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UniProt

P40967

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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