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Description
Images & Validation
−
Item 1 of 6
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
| Reactivity | Human, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | 3D3 |
| Immunogen | A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days