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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.7&36.8 KDa. Observed: 40-55&20 KDa, reducing conditions
Mouse
Val22-Ala196&Met23-Ser335
C-6His
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37 KDa. Observed: 60 KDa, reducing conditions
Human
Asp20-Tyr337
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 50.4 KDa. Observed: 28-50&70 KDa, reducing conditions
Mouse
Glu22-Glu448
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 47.9 KDa. Observed: 58-60 KDa, reducing conditions
Mouse
Ala23-Ile436
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 61 KDa. Observed: 80-120 KDa, reducing conditions
Mouse
Trp39-Asn332
C-Fc-6His
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 7.8 KDa. Observed: 12—14 KDa, reducing conditions
Human
Ala30-Arg88
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 95 KDa. Observed: 120 KDa, reducing conditions
Human
Lys19-Arg638
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 53.5 KDa. Observed: 90 KDa, reducing conditions
Human
Met1-Ser486
C-6His
500 μg, 1 mg, 10 μg, 50 μgGreater than 90% as determined by reducing SDS-PAGE.
Predicted: 62.6 KDa. Observed: 67-81 KDa, reducing conditions
Human
Gln27-Ala349
C-Fc
1 mgUnconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 43.6 KDa. Observed: 52 KDa, reducing conditions
Human
Gln45-Pro193
C-Fc
10 μg, 50 μg, 500 μg, 1 mg
