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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.3 KDa. Observed: 58-66 KDa, reducing conditions
Human
Met1-Ala537
N, C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15 KDa. Observed: 14 KDa, reducing conditions
Human
Met1-Phe140
Tag free
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 39.4 KDa. Observed: 56 KDa, reducing conditions
Human
Phe20-Arg356
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.9 KDa. Observed: 40-50 KDa, reducing conditions
Human
Thr20-Leu384
Tag free
500 μg, 1 mg, 10 μg, 50 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 29.3 KDa. Observed: 45-85 KDa, reducing conditions
Human
Leu23-Ala262
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.9 KDa. Observed: 19 KDa, reducing conditions
Human
Gln282-Ser433
N-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 10.6 KDa. Observed: 10—14 KDa, reducing conditions
Human
Lys19-Pro108
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 24 KDa, reducing conditions
Human
Met1-Gly76
N-6His, SUMO tag
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.7 KDa. Observed: 42 KDa, reducing conditions
Mouse
Ala20-Phe374
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.4 KDa. Observed: 30 KDa, reducing conditions
Human
Met1-Lys162
N, C-6His
10 μg, 50 μg, 500 μg, 1 mg
