Protein Molecular Weight Marker

SKU: orb1567188

Description

Images & Validation

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Item 1 of 3

Tested Applications

SDS-PAGE

Application Notes

Protein Molecular Weight Marker has been tested and is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats

Key Properties

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Purity

Protein Molecular Weight Marker is supplied in 1X loading buffer and is ready-to-use. It contains: [62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue.] This product are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference.

Conjugation

Unconjugated

Storage & Handling

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Storage	Store vial at -20° C.
Form/Appearance	Liquid (in 1x Loading Buffer)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 2.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
Concentration	1X
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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Protein ladder, Protein marker, Protein standard, Protein molecular weight standard, Molecular weight marker, Protein molecular weight ladder, Protein marker, Mw marker, Mw standard

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

SDS PAGE of Protein Molecular Weight Marker, 14-120 kDa

SDS PAGE Results of Protein Molecular Weight Marker 14-120kDa. Lane 1: Opal Prestained Molecular Weight Marker (11-245kDa). [7 µl] Lane 2: Protein Molecular Weight Marker (14-120kDa) (p/n orb348606). [5 µl] 4-20% Gel, Coomassie Stained.

SDS-Page of Protein Molecular Weight Marker. Lane 1: Protein Molecular Weight Marker 12.5 uL. Lane 2: Protein Molecular Weight Marker 10.0 uL. Lane 3: Protein Molecular Weight Marker 7.5 uL. Lane 4: Protein Molecular Weight Marker 5.0 uL. Exact molecular weights are detailed on image 1.

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Protocol Information

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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