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PRPF8 Antibody
SKU: orb1257215
Featured
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). |
| Target | PRPF8 |
| Molecular Weight | Observed: 274kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HPRP8, PRP8, PRPC8, RP13, SNRNP22pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p22precursor mRNA processing protein, splicing factor Prp8
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Western blot analysis of extracts of various cell lines, using PRPF8 antibody (orb1257215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (orb1257215) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
PRPF8
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
PRPF8 Antibody (orb1257215)
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