Recombinant PSA Antibody / Prostate Specific Antigen

SKU: orb2642160

Description

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with mAb A67-B/E3 for PSA tests.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	The optimal dilution of the recombinant PSA antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KLK3/2871R
Immunogen	Recombinant human protein was used as the immunogen for this recombinant PSA antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant PSA Antibody / Prostate Specific Antigen

IHC staining of FFPE human prostate carcinoma with PSA antibody (clone KLK3/2871R). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PSA antibody (clone KLK3/2871R) as confirmation of integrity and purity.

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UniProt

P07288

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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