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Purmorphamine

SKU: orb1224975

Description

Purmorphamine, which directly binds and activates Smoothened, blocks BODIPY-cyclopamine binding to Smo with IC50 of ~ 1.5 μM in HEK293T cell and also is an inducer of osteoblast differentiation with EC50 of 1 μM.

Images & Validation

Key Properties

CAS Number483367-10-8
MW520.62
Purity>98% (HPLC)
FormulaC31H32N6O2
SMILESN1(C2CCCCC2)C=NC3=C(NC4=CC=C(N5CCOCC5)C=C4)N=C(OC6=C7C=CC=CC7=CC=C6)N=C13
TargetHedgehog (Hh)
SolubilityDMSO: 4 mg/mL warmed (7.68 mM)

Bioactivity

In Vitro
Purmorphamine (GMP) (1.5 μM, 1 week) can generate embryoid bodies (EBs) from human iPS cells (hiPS2) and hESCs (HSF1). Purmorphamine (GMP) (1.5 μM) converts hPSCs to NKX2-1 positive MGE cells. Purmorphamine (GMP) (2 μM, day 28-35) promotes ventralization of the brain spheroids in differentiation process from hiPSCs. Purmorphamine (GMP) (1 μM) together with retinoic acid (RA, 2 μM) promotes hiPSC differentiation into functional neural tissue, indicated by the expression of neuronal marker β-tubulin III (βT-III). Purmorphamine (GMP) (day5-7) induces hESCs differentiation into dopaminergic (DA) neurons.

Storage & Handling

StorageStorage temperature: -20°C. Stability: ≥ 2 years
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Purmorphamine

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    99.48% (May vary between batches)

    483367-10-8

    520.62

    C31H32N6O2

    2 mg, 5 mg, 10 mg, 50 mg, 1 ml x 10 mM (in DMSO), 1 mg, 200 mg, 25 mg, 100 mg
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Purmorphamine (orb1224975)

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100 mg
200 mg
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2 mg
$ 100.00
5 mg
$ 140.00
10 mg
$ 220.00
50 mg
$ 410.00