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Rabbit anti-53BP1 Recombinant Monoclonal Antibody
SKU: orb1519978
Description
Images & Validation
−Item 1 of 6
| Tested Applications | FC, IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BL-250-1H11 |
| Immunogen | Between 350 and 400 |
| Target | 53BP1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−p53-binding protein 1; tumor suppressor p53-binding protein 1; tumor protein 53-binding protein, 1; TP53-binding protein 1; p53BP1; p202; 53BP1; TDRD30
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Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK) , and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 second.
Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used for IP at 20 µl per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005648.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IP
Immunoprecipitation
Rabbit anti-53BP1 Recombinant Monoclonal Antibody (orb1519978)
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