Rabbit 53BP1 Recombinant Monoclonal Antibody

SKU: orb1519978

Description

53BP1 Antibody

Images & Validation

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Item 1 of 6

Tested Applications	FC, IHC, IP, WB
Dilution Range	WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BL-250-1H11
Immunogen	Between 350 and 400
Target	53BP1
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	100 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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53BP1; p202; p53-binding protein 1; p53BP1; TDRD30; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1

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Rabbit 53BP1 Recombinant Monoclonal Antibody

Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK) , and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 second.

Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used for IP at 20 µl per reaction.

Quick Database Links

Gene Symbol

53BP1

UniProt

Q12888

RefSeq (Protein):NP_005648.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005648.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation