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Rabbit ARID1A/BAF250 Recombinant Monoclonal Antibody
SKU: orb1806460
Description
Research Area
Cancer Biology, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 9
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 20 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 (3-8% Tris-acetate gel recommended) |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR279L |
| Immunogen | Between 750 and 800 |
| Target | ARID1A/BAF250 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 50 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−ARID domain-containing protein 1A;AT rich interactive domain 1A (SWI-like);AT-rich interactive domain-containing protein 1A;B120;BAF250;BAF250a;BM029;brain protein 120;BRG1-associated factor 250;BRG1-associated factor 250a;C1orf4;chromatin remodeling factor p250;CSS2;ELD;hELD;hOSA1;MRD14;osa homolog 1;OSA1;OSA1 nuclear protein;P270;SMARCF1;SWI/SNF complex protein p270;SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1;SWI-like protein
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Rabbit ARID1A/BAF250 Recombinant Monoclonal Antibody [orb1806459]
FC, ICC, IHC, IP, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μg (BSA-free)
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Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used for IP at 20 µl/mg lysate.
Detection of mouse ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of mouse mIMCD-3 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006006.3 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit ARID1A/BAF250 Recombinant Monoclonal Antibody (orb1806460)
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