Rabbit ASAP1 Antibody

SKU: orb1527186

Description

ASAP1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 700 and 750
Target	ASAP1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP1; ARF GTPase-activating protein 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin, beta 4; CENTB4; DDEF1; DEF-1; development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; PAG2; PAP; PIP2-dependent ARF1 GAP; ZG14P

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Detection of human ASAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ASAP1 antibody orb1527186 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

ASAP1

UniProt

Q9ULH1

RefSeq (Protein):NP_060952.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_060952.2

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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