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Rabbit B7-H6 Recombinant Monoclonal Antibody
SKU: orb1784569
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IP, WB |
|---|---|
| Dilution Range | Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR271L |
| Immunogen | Residues 25-262 (ECD) |
| Target | B7-H6 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B7 homolog 6;B7H6;B7-H6;DKFZp686O24166;natural cytotoxicity triggering receptor 3 ligand 1;putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
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Detection of human B7-H6 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, K-562, Hep-G2, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human B7-H6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used for IP at 6 µl/mg lysate
Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of Hep-G2 cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of HEK293T cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001189368.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit B7-H6 Recombinant Monoclonal Antibody (orb1784569)
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