Rabbit BIG2/ARFGEF2 Antibody

SKU: orb1529238

Description

BIG2/ARFGEF2 Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 300 and 350
Target	BIG2/ARFGEF2
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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ADP-ribosylation factor guanine nucleotide-exchange factor 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited); BIG2; brefeldin A-inhibited GEP 2; brefeldin A-inhibited guanine nucleotide-exchange protein 2; dJ1164I10.1; PVNH2

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Detection of human BIG2/ARFGEF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 used at a dilution of 1:200 (1µg/ml).

Detection of human BIG2/ARFGEF2 by WB and IP.Samples: Whole cell lysate from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody orb1529238 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).

Quick Database Links

Gene Symbol

BIG2/ARFGEF2

UniProt

Q9Y6D5

RefSeq (Protein):NP_006411.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006411.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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