Rabbit BRD2 Antibody

SKU: orb1526370

Description

BRD2 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; ICC-IF - 1:50 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Reactivity	Human
Predicted Reactivity	Bovine
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	BRD2
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3

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Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody orb1526370 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody orb1526370 used for IP at 6 µg per reaction.

Detection of human BRD2 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.orb1526370) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

BRD2

UniProt

P25440

RefSeq (Protein):NP_005095.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005095.1

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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Immunoprecipitation

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