Rabbit BRD7 Antibody

SKU: orb1526866

Description

BRD7 Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC, IP, WB
Dilution range	WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 601 and C-terminus
Target	BRD7
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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75 kDa bromodomain protein; BP75; bromodomain-containing protein 7; CELTIX1; NAG4; protein CELTIX-1

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Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody orb1526866 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody orb1526866 used for IP at 6 µg per reaction.

Detection of human BRD7 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (Cat. No. orb1526866) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human BRD7 (shaded) in HeLa cells by flow cytometry.Antibody: Rabbit anti-BRD7 polyclonal antibody (orb1526866)) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse BRD7 (shaded) in EL4 cells by flow cytometry.Antibody: Rabbit anti-BRD7 polyclonal antibody (orb1526866)) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

BRD7

UniProt

Q9NPI1

RefSeq (Protein):NP_037395.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_037395.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation