Rabbit c-Myb Antibody

SKU: orb1523550

Description

c-Myb Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP
Dilution Range	WB - Not recommended. Use rabbit anti-c-Myb antibody A304-138A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	c-Myb
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cmyb; c-myb; c-myb_CDS; efg; oncogene AMV; proto-oncogene c-Myb; transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog

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Detection of human c-Myb by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT-4 cells. Antibodies: Affinity purified rabbit anti-c-Myb antibody orb1523550 used for IP at 6 µg/mg lysate.

Detection of mouse c-Myb by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human c-Myb by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

c-Myb

UniProt

P10242

RefSeq (Protein):NP_005366.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005366.2

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Protocol Information

IHC

Immunohistochemistry

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Immunoprecipitation

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