Rabbit anti-CD3E Recombinant Monoclonal Antibody

SKU: orb1519964

Featured

Description

CD3E Antibody

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this antibody works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

1 Results

Dilution

Sample

Summary

IHC

Human

1:250

Ormalin-fixed paraffin-embedded (FFPE) normal intestinal tissue and immune-related enterocolitis (irEC) tissue biopsies

The antibody was used on human...

Images & Validation

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Item 1 of 6

Tested Applications	FC, IF, IHC, IP, WB, mIF
Dilution range	WB 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 - 1:500, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cell. mIF: 1:250
Reactivity	Human
Application Notes	All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BL-298-5D12
Immunogen	Between 157 and C-terminus
Target	CD3E
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Disclaimer	For research use only

Alternative Names

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CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T3E

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rabbit anti-CD3E Recombinant Monoclonal Antibody

Detection of human CD3E by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH

Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human CD3E by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal™. Counterstain: DAPI (blue)

Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CD3E recombinant monoclonal antibody (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG

Detection of human CD3E in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD3E Recombinant Monoclonal Antibody (orb1519964) Secondary: Goat anti-Rabbit IgG Heavy and Light Chain Highly Cross-Adsorbed Antibody (HRP) (orb1531012). Substrate: DAB.

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766

RefSeq (Protein):NP_000724.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000724.1

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence

Immunoprecipitation