Rabbit anti-CD4 Recombinant Monoclonal Antibody

SKU: orb1519966

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Description

CD4 Antibody

Images & Validation

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Tested Applications	FC, IF, IHC, IP, WB
Dilution range	Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 6 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Reactivity	Human
Application Notes	All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BL-155-1C11
Immunogen	Residues 26-397 (ECD)
Target	CD4
Purity	Recombinant antibody was purified from cell culture supernatant
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; CD antigen CD4; CD4 antigen (p55); CD4 receptor

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Rabbit anti-CD4 Recombinant Monoclonal Antibody

Detection of human CD4 by western blot. Samples: Whole cell lysate (50 µg) from U-937, HEK293T, MOLT-4, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody [BL-155-1C11] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human CD4 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human CD4 (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG

Quick Database Links

Gene Symbol

CD4

UniProt

P01730

RefSeq (Protein):NP_000607.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000607.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence

Immunoprecipitation