Rabbit CD48 Recombinant Monoclonal Antibody

SKU: orb1806447

Description

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	FC, IP, WB
Dilution Range	Application : Flow-Cyt , ApplicationDilutionRange : Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : IP , ApplicationDilutionRange : 20 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR286L
Immunogen	Residues 29-220 (ECD)
Target	CD48
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	100 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BCM1;BCM1 surface antigen;BLAST;BLAST1;B-lymphocyte activation marker BLAST-1;CD antigen CD48;CD48 antigen;CD48 antigen (B-cell membrane protein);hCD48;leukocyte antigen MEM-102;mCD48;MEM-102;signaling lymphocytic activation molecule 2;SLAM family member 2;SLAMF2;TCT.1

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Rabbit CD48 Recombinant Monoclonal Antibody

Detection of human CD48 by western blot. Samples: Whole cell lysate (10 µg) from Raji, Jurkat, Ramos, CAL-27, and NK-92 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1806447) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD48 (shaded) in NK-92 cells by flow cytometry. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1806447) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human CD48 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD48 recombinant monoclonal antibody (orb1806447) used for IP at 20 µl/mg lysate.

Quick Database Links

Gene Symbol

CD48

UniProt

P09326

RefSeq (Protein):NP_001769.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001769.2

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation