Rabbit CHMP2B Antibody

SKU: orb1522885

Description

CHMP2B Antibody

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution range	WB - 1:1,000 - 1:5,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Frog, Gallus, Xenopus
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 163 and C-terminus
Target	CHMP2B
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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ALS17; charged multivesicular body protein 2b; CHMP2.5; CHMP2b; chromatin modifying protein 2B; Chromatin-modifying protein 2b; DMT1; hVps2-2; Vacuolar protein sorting-associated protein 2-2; vacuolar protein-sorting-associated protein 2-2; VPS2 homolog B; VPS2-2; VPS2B

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Detection of human and mouse CHMP2B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 and (20 µg) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHMP2B antibody orb1522885 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CHMP2B by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHMP2B (Cat. No. orb1522885) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Quick Database Links

Gene Symbol

CHMP2B

UniProt

Q9UQN3

RefSeq (Protein):NP_054762.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_054762.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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