Rabbit DDX6 Antibody

SKU: orb1530165

Description

DDX6 Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:25,000 - 1:50,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Guinea pig
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 433 and C-terminus
Target	DDX6
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); HLR2; oncogene RCK; P54; probable ATP-dependent RNA helicase DDX6; RCK

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Detection of human DDX6 by western blot.Samples: Whole cell lysate from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-DDX6 antibody orb1530165 at 0.04 µg/ml.

Detection of mouse DDX6 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-DDX6 antibody orb1530165 at 0.04 µg/ml.

Detection of human DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human DDX6 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody.

Quick Database Links

Gene Symbol

DDX6

UniProt

P26196

RefSeq (Protein):NP_004388.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004388.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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