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Rabbit E-Cadherin Recombinant Monoclonal Antibody
SKU: orb1519779
Description
Research Area
Cancer Biology, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR088G |
| Immunogen | Residues 155-707 (ECD) |
| Target | E-Cadherin |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Arc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD antigen CD324; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin
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Detection of human E-Cadherin in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (orb1519779). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (orb1519779) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004351.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit E-Cadherin Recombinant Monoclonal Antibody (orb1519779)
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