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Rabbit ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody
SKU: orb1530835
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, ICC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate; ICC - 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:75,000; for coating plates 1:100 - 1:500 |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLRE00G |
| Immunogen | Enterokinase Cleavage Site (DYKDDDDK) |
| Target | ECS (DYKDDDDK) Tag |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of ECS (DYKDDDDK)-tagged protein by WB from non-transfected HEK293 cells, HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1, IDO1-ECS(DYKDDDDK)-HIS (Internal Tag), IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag), and SUP-M2 cells, at 1:1000.
Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag) lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.
Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1 lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.
Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing ECS (DYKDDDDK)-tagged nuclear protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.
Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing an ECS (DYKDDDDK)-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.
Quick Database Links
Gene Symbol
ECS (DYKDDDDK) Tag
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Rabbit ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody (orb1530835)
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