Rabbit FANCA Antibody

SKU: orb1527444

Description

FANCA Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 3

Tested Applications	FC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1405 and C-terminus
Target	FANCA
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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FA; FA1; FAA; FACA; FAH; FA-H; FANCH; Fanconi anemia complementation group A; Fanconi anemia group A protein; Fanconi anemia, complementation group H; Fanconi anemia, type 1; Protein FACA

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Detection of human FANCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCA antibody (orb1527444) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human FANCA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCA antibody (orb1527444) used for IP at 6 µg per reaction.

Detection of human FANCA (shaded) in HDLM-2 cells by flow cytometry.Antibody: Rabbit anti-FANCA polyclonal antibody (orb1527444) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

FANCA

UniProt

O15360

RefSeq (Protein):NP_000126.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000126.2

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation