Rabbit FOXP3 Recombinant Monoclonal Antibody

SKU: orb1519916

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Description

FOXP3 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Tested Applications	ICC, IF, IHC, IP, WB
Dilution Range	Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Reactivity	Human
Application Notes	All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR034F
Immunogen	Between 275 and 325
Target	FOXP3
Purity	Recombinant antibody was purified from cell culture supernatant
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AIID; DIETER; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; PIDX; scurfin; XPID

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Rabbit FOXP3 Recombinant Monoclonal Antibody

Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody

Detection of human FOXP3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human FOXP3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human FOXP3 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue)

Quick Database Links

Gene Symbol

FOXP3

UniProt

Q9BZS1

RefSeq (Protein):NP_054728.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_054728.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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