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Rabbit anti-GFP Recombinant Monoclonal Antibody
SKU: orb1784590
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IP, WB |
|---|---|
| Dilution range | WB: 1:1000, IP: 20 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLRE04L |
| Immunogen | Green Fluorescent Protein |
| Target | GFP |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 50 µg/ml |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Detection of GFP by western blot.Samples: Whole cell lysate from HEK293T cells transfected with EGFP-KAP1, KAP1-EGFP, or untransfected. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.
Detection of GFP by western blot.Samples: Whole cell lysate from 293T cells transfected with EGFP, YP, CFP, AcGFP, or BFP. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.
Detection of GFP by western blot of immunoprecipitates.Samples: Whole cell lysate from 293-F cells tranfected with EGFP-KAP1. Antibodies: Rabbit anti-GFP recombinant monoclonal antibody.
Detection of GFP by ICC. Sample: FFPE section of HEK293 cells expressing a GFP nuclear protein (left) and untransfected HEK293 cells (right). Antibody: Rabbit anti-GFP recombinant monoclonal antibody.
Quick Database Links
Gene Symbol
GFP
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit anti-GFP Recombinant Monoclonal Antibody (orb1784590)
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