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Rabbit HA Tag Recombinant Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, ICC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500 |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLRE02G |
| Immunogen | HA Epitope tag (YPYDVPDYA) |
| Target | HA Tag |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of HA-tagged protein by WB from HEK293 transfected with Met-HA-IDO1, HEK293 with IDO1-HA-V5 (Internal Tag), and HEK293 with IDO1-V5-HA (C-terminal Tag) cells, using Rabbit anti-HA Tag recombinant monoclonal antibody at 1:1000.

Detection of HA Tag by western blot of immunoprecipitates from HEK293 transfected with Met-HA-IDO1 lysate.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody and Rabbit anti-IDO1 recombinant monoclonal antibody .

Detection of HA Tag in FFPE HEK293 cells expressing HA tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody.

Detection of HA Tag in FFPE HEK293 cells expressing HA tagged nuclear protein by ICC.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody.
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Rabbit HA Tag Recombinant Monoclonal Antibody (orb1530833)
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